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CATH Superfamily 3.10.110.10

Ubiquitin Conjugating Enzyme

The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was named:

"
Ubiquitin Conjugating Enzyme
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 9: Ubiquitin-conjugating enzyme E2 R2

There are 2 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
E2 ubiquitin-conjugating enzyme. [EC: 2.3.2.23]
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
  • The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself.
  • In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage.
  • Formerly EC 6.3.2.19.
 1881 A0A024R1Z1 A0A024R1Z1 A0A024R1Z1 A0A024R1Z1 A0A091DT64 A0A091DT64 A0A091DT64 A0A091DT64 A0A091DT64 A0A091DT64
(1871 more...)
(E3-independent) E2 ubiquitin-conjugating enzyme. [EC: 2.3.2.24]
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)- monoubiquitinyl-[acceptor protein]-L-lysine.
  • The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27).
  • It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg(2+)-ATP and catalyzes the conjugation of ubiquitin to protein substrates, independently of E3.
  • This transfer has only been observed with small proteins.
  • In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L- lysine methyl ester) has been observed.
 32 A0A024R1Z1 A0A024R1Z1 A0A024R1Z1 A0A024R1Z1 A0A1A6H8Z5 A0A1A6H8Z5 A0A1A6H8Z5 A0A1A6H8Z5 A0A2J8R0Q4 A0A2J8R0Q4
(22 more...)