The process in which one or more ubiquitin groups are added to a protein.

The process of directing proteins towards the vacuole that contributes to protein catabolism via the multivesicular body (MVB) pathway.

The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of a ubiquitin group, or multiple ubiquitin groups, to the protein.

The process in which one or more ubiquitin groups are added to a protein.

The process in which one or more ubiquitin groups are added to a protein.

A process in which a protein is transported to, or maintained in, a location within the Golgi apparatus.

The process of directing proteins towards the vacuole that contributes to protein catabolism via the multivesicular body (MVB) pathway.

Any process that modulates the internal pH of a cell, measured by the concentration of the hydrogen ion.

A protein modification process in which one or more groups of a small protein, such as ubiquitin or a ubiquitin-like protein, are covalently attached to or removed from a target protein.

A pathway targeting soluble cytosolic proteins to the vacuole lumen. It uses a selective autophagy receptor protein Nbr1, which is an ortholog of mammalian NBR1, and is remotely related to S. cerevisiae Cvt pathway receptor protein Atg19. Similar to the Cvt pathway, the cargos transported by this pathway are hydrolases, which presumably contribute to the hydrolytic activities in the vacuole lumen. Different from the Cvt pathway, this pathway does not require the macroautophagy machinery, but instead relies on the ESCRT machinery for cargo sequestration. This pathway is observed in the fission yeast S. pombe.

Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to plasma membrane.

Any process that stops, prevents or reduces the frequency, rate or extent of uracil import across plasma membrane.

Any process that stops, prevents or reduces the frequency, rate or extent of leucine import across plasma membrane.

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

A compound membranous cytoplasmic organelle of eukaryotic cells, consisting of flattened, ribosome-free vesicles arranged in a more or less regular stack. The Golgi apparatus differs from the endoplasmic reticulum in often having slightly thicker membranes, appearing in sections as a characteristic shallow semicircle so that the convex side (cis or entry face) abuts the endoplasmic reticulum, secretory vesicles emerging from the concave side (trans or exit face). In vertebrate cells there is usually one such organelle, while in invertebrates and plants, where they are known usually as dictyosomes, there may be several scattered in the cytoplasm. The Golgi apparatus processes proteins produced on the ribosomes of the rough endoplasmic reticulum; such processing includes modification of the core oligosaccharides of glycoproteins, and the sorting and packaging of proteins for transport to a variety of cellular locations. Three different regions of the Golgi are now recognized both in terms of structure and function: cis, in the vicinity of the cis face, trans, in the vicinity of the trans face, and medial, lying between the cis and trans regions.

The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures.