CATH Classification

Domain Context

CATH Clusters

Superfamily Hotdog Thioesterase
Functional Family 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Enzyme Information

5.3.3.14
Trans-2-decenoyl-[acyl-carrier-protein] isomerase.
based on mapping to UniProt P0A6Q3
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier- protein].
-!- While the enzyme from Escherichia coli is highly specific for the 10-carbon enoyl-ACP, the enzyme from Streptococcus pneumoniae can also use the 12-carbon enoyl-ACP as substrate in vitro but not 14- or 16-carbon enoyl-ACPs. -!- ACP can be replaced by either CoA or N-acetylcysteamine thioesters. -!- The cis-3-enoyl product is required to form unsaturated fatty acids, such as palmitoleic acid and cis-vaccenic acid, in dissociated (or type II) fatty-acid biosynthesis.
4.2.1.59
3-hydroxyacyl-[acyl-carrier-protein] dehydratase.
based on mapping to UniProt P0A6Q3
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O.
-!- This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. -!- The enzyme uses fatty acyl thioesters of ACP in vivo. -!- Different forms of the enzyme may have preferences for substrates with different chain length. -!- For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. -!- Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyzes EC 5.3.3.14. -!- Despite the differences both forms can catalyze all steps leading to the synthesis of palmitate (C16:0). -!- FabZ, but not FabA, can also accept unsaturated substrates. -!- Formerly EC 4.2.1.58, EC 4.2.1.60 and EC 4.2.1.61.

UniProtKB Entries (1)

P0A6Q3
FABA_ECOLI
Escherichia coli K-12
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

PDB Structure

PDB 4KEH
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Trapping the dynamic acyl carrier protein in fatty acid biosynthesis.
Nguyen, C., Haushalter, R.W., Lee, D.J., Markwick, P.R., Bruegger, J., Caldara-Festin, G., Finzel, K., Jackson, D.R., Ishikawa, F., O'Dowd, B., McCammon, J.A., Opella, S.J., Tsai, S.C., Burkart, M.D.
Nature