CATH Classification

Domain Context

CATH Clusters

Superfamily Aldolase class I
Functional Family Pyruvate formate-lyase-activating enzyme

Enzyme Information

1.97.1.4
[Formate-C-acetyltransferase]-activating enzyme.
based on mapping to UniProt P0A9N4
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.
-!- A single glycine residue in EC 2.3.1.54 is oxidized to the corresponding radical by transfer of H from its CH(2) to AdoMet with concomitant cleavage of the latter. -!- The first stage is reduction of the AdoMet to give methionine and the 5'-deoxyadenosin-5'-yl radical, which then abstracts a hydrogen radical from the glycine residue.

UniProtKB Entries (1)

P0A9N4
PFLA_ECOLI
Escherichia coli K-12
Pyruvate formate-lyase 1-activating enzyme

PDB Structure

PDB 3CB8
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme.
Vey, J.L., Yang, J., Li, M., Broderick, W.E., Broderick, J.B., Drennan, C.L.
Proc.Natl.Acad.Sci.Usa