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CATH Superfamily 1.10.10.10

Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

The name of this superfamily has been modified since the most recent official CATH+ release (v4_2_0). At the point of the last release, this superfamily was named:

"
Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 266417: O-methyltransferase family protein, expressed

There are 34 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
Caffeate O-methyltransferase. [EC: 2.1.1.68]
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L- homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
  • 3,4-dihydroxybenzaldehyde and catechol can act as acceptor, more slowly.
 144 A0A068U9K4 A0A068U9K4 A0A0B2NUU9 A0A0B2NUU9 A0A0B2NZX2 A0A0B2NZX2 A0A0B2NZY1 A0A0B2NZY1 A0A0B2Q0S8 A0A0B2Q0S8
(134 more...)
Quercetin 3-O-methyltransferase. [EC: 2.1.1.76]
S-adenosyl-L-methionine + 3,5,7,3',4'-pentahydroxyflavone = S-adenosyl-L- homocysteine + 3-methoxy-5,7,3',4'-tetrahydroxyflavone.
  • Specific for quercetin.
  • Related enzymes bring about the 3-O-methylation of other flavonols, such as galangin and kaempferol.
 102 A0A0B2NPP8 A0A0B2NPP8 A0A0B2NUU9 A0A0B2NUU9 A0A0B2NZX2 A0A0B2NZX2 A0A0B2NZY1 A0A0B2NZY1 A0A0B2P6P4 A0A0B2P6P4
(92 more...)
Catechol O-methyltransferase. [EC: 2.1.1.6]
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.
  • The mammalian enzymes act more rapidly on catecholamines such as adrenaline or noradrenaline than on catechols.
 54 A0A0A6Z5Z8 A0A0A6Z5Z8 A0A0A6Z5Z9 A0A0A6Z5Z9 A0A0A6Z653 A0A0A6Z653 A0A0X8TVH5 A0A0X8TVH5 B6E624 B6E624
(44 more...)
(Iso)eugenol O-methyltransferase. [EC: 2.1.1.146]
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol.
  • Acts on eugenol and chavicol as well as isoeugenol.
 44 A0A1B6PFT6 A0A1B6PFT6 A8QW52 A8QW52 B9RGB0 B9RGB0 B9RVQ8 B9RVQ8 B9RVR2 B9RVR2
(34 more...)
Acetylserotonin O-methyltransferase. [EC: 2.1.1.4]
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.
  • Some other hydroxyindoles also act as acceptor, more slowly.
 32 A0A0E0QEJ7 A0A0E0QEJ7 A0A191B549 A0A191B549 B3GSH5 B3GSH5 D2KCH8 D2KCH8 D3KU66 D3KU66
(22 more...)
O-demethylpuromycin O-methyltransferase. [EC: 2.1.1.38]
S-adenosyl-L-methionine + O-demethylpuromycin = S-adenosyl-L-homocysteine + puromycin.
  • Puromycin is the antibiotic derived from N(6)-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.
 24 A0A0T9SCW0 A0A0T9SCW0 A0A0T9UQS4 A0A0T9UQS4 A0A1B7JFU2 A0A1B7JFU2 A0A1E4QHI8 A0A1E4QHI8 A0A1L7EQ28 A0A1L7EQ28
(14 more...)
3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase. [EC: 2.1.1.116]
S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine = S-adenosyl-L-homocysteine + (S)-reticuline.
  • Involved in isoquinoline alkaloid metabolism in plants.
  • Has also been shown to catalyze the methylation of (RS)- laudanosoline, (S)-3'-hydroxycoclaurine and (RS)-7- O-methylnoraudanosoline.
 14 A0A0B4VG66 A0A0B4VG66 A0A0B4VGZ0 A0A0B4VGZ0 B9SUX8 B9SUX8 Q5C9L4 Q5C9L4 Q7XB10 Q7XB10
(4 more...)
Isoflavone 4'-O-methyltransferase. [EC: 2.1.1.46]
S-adenosyl-L-methionine + a 4'-hydroxyisoflavone = S-adenosyl-L- homocysteine + a 4'-methoxyisoflavone.
  • The enzyme catalyzes the methylation of daidzein and genistein.
  • It does not methylate naringenin, apigenin, luteolin or kaempferol.
 14 A0A072UNK8 A0A072UNK8 I3RZV3 I3RZV3 K9K000 K9K000 O24305 O24305 Q29U70 Q29U70
(4 more...)
(RS)-norcoclaurine 6-O-methyltransferase. [EC: 2.1.1.128]
S-adenosyl-L-methionine + (RS)-norcoclaurine = S-adenosyl-L-homocysteine + (RS)-coclaurine.
  • The enzyme will also catalyze the 6-O-methylation of (RS)- norlaudanosoline to form 6-O-methyl-norlaudanosoline, but this alkaloid has not been found to occur in plants.
 12 A0A0B4VG62 A0A0B4VG62 A0A0B4VGY7 A0A0B4VGY7 B9SUY0 B9SUY0 Q5C9L7 Q5C9L7 Q6WUC1 Q6WUC1
(2 more...)
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase. [EC: 2.1.1.212]
S-adenosyl-L-methionine + 2,4',7-trihydroxyisoflavanone = S-adenosyl-L- homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone.
  • Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position.
  • No activity with isoflavones.
  • The enzyme is involved in formononetin biosynthesis in legumes.
  • The protein from pea (Pisum sativum) also methylates (+)- 6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270).
 10 A0A072UNK8 A0A072UNK8 I3RZV3 I3RZV3 Q29U70 Q29U70 Q84KK4 Q84KK4 Q84KK6 Q84KK6
Isoflavone 7-O-methyltransferase. [EC: 2.1.1.150]
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L- homocysteine + a 7-methoxyisoflavone.
  • The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
 10 B9S9B7 B9S9B7 O22308 O22308 O22309 O22309 O24529 O24529 Q84KK5 Q84KK5
5-hydroxyfuranocoumarin 5-O-methyltransferase. [EC: 2.1.1.69]
(1) S-adenosyl-L-methionine + a 5-hydroxyfurocoumarin = S-adenosyl-L- homocysteine + a 5-methoxyfurocoumarin. (2) S-adenosyl-L-methionine + bergaptol = S-adenosyl-L-homocysteine + bergapten.
  • Converts bergaptol into bergapten, which has therapeutic potential in the treatment of psoriasis as it has photosensitizing and antiproliferative activities.
  • Methylates the 5-hydroxy group of some hydroxy- and methylcoumarins, such as 5-hydroxyxanthotoxin, but has little activity on non-coumarin phenols; caffeate, 5-hydroxyferulate and daphnetin are not substrates.
  • Cu(2+), Zn(2+) and Co(2+) cause enzyme inhibition.
  • See also EC 2.1.1.70.
  • Formerly EC 2.1.1.92.
 8 A0A166U5H3 A0A166U5H3 A8J6X1 A8J6X1 J9PHM5 J9PHM5 Q6T1F6 Q6T1F6
Flavone 3'-O-methyltransferase. [EC: 2.1.1.42]
S-adenosyl-L-methionine + 3'-hydroxyflavone = S-adenosyl-L-homocysteine + 3'-methoxyflavone.
  • The enzyme prefers flavones with vicinal 3',4'-dihydroxyl groups.
 6 A0A0E0QEJ7 A0A0E0QEJ7 Q6ZD89 Q6ZD89 Q9FK25 Q9FK25
(S)-scoulerine 9-O-methyltransferase. [EC: 2.1.1.117]
S-adenosyl-L-methionine + (S)-scoulerine = S-adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine.
  • The product of this reaction is a precursor for protoberberine alkaloids in plants.
 4 Q39522 Q39522 Q5C9L2 Q5C9L2
Anthranilate N-methyltransferase. [EC: 2.1.1.111]
S-adenosyl-L-methionine + anthranilate = S-adenosyl-L-homocysteine + N-methylanthranilate.
  • Involved in the biosynthesis of acridine alkaloids in plant tissues.
 4 A9X7L0 A9X7L0 M4HXR6 M4HXR6
5-pentadecatrienyl resorcinol O-methyltransferase. [EC: 2.1.1.n7]
S-adenosyl-L-methionine + (8Z,11Z)-5-(pentadeca-8,11,14-trien-1- yl)resorcinol = S-adenosyl-L-homocysteine + (8Z,11Z)-5-(pentadeca- 8,11,14-trien-1-yl)resorcinol-3-methyl ether.
  • In Sorghum, this enzyme is part of the biosynthetic pathway of the phytotoxin sorgoleone.
 4 A8QW53 A8QW53 C5YBN8 C5YBN8
Calcium-transporting ATPase. [EC: 3.6.3.8]
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2).
  • P-type ATPase that undergoes covalent phosphorylation during the transport cycle.
  • This enzyme family comprises three types of Ca(2+)-transporting enzymes that are found in the plasma membrane, the sarcoplasmic reticulum and in Saccharomyces cerevisiae.
  • The first and third transport one ion per ATP hydrolyzed, whereas the second transports two ions.
  • Formerly EC 3.6.1.38.
 4 K7TI47 K7TI47 K7TWC7 K7TWC7
(+)-6a-hydroxymaackiain 3-O-methyltransferase. [EC: 2.1.1.270]
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain = S-adenosyl-L- homocysteine + (+)-pisatin.
  • The protein from the plant Pisum sativum (garden pea) methylates (+)- 6a-hydroxymaackiain at the 3-position.
  • It also methylates 2,7,4'-trihydroxyisoflavanone on the 4'-position (cf. EC 2.1.1.212).
 4 O24305 O24305 P0DH60 P0DH60
Tabersonine 16-O-methyltransferase. [EC: 2.1.1.94]
S-adenosyl-L-methionine + 16-hydroxytabersonine = S-adenosyl-L- homocysteine + 16-methoxytabersonine.
  • Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle, Catharanthus roseus.
 2 B0EXJ8 B0EXJ8
Inositol 4-methyltransferase. [EC: 2.1.1.129]
S-adenosyl-L-methionine + myo-inositol = S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol.
  • The enzyme from Vigna umbellata is highly specific for S-adenosyl-L- methionine.
  • The enzyme also methylates 1L-1,2,4/3,5-cyclohexanepentol, 2,4,6/3,5- pentahydroxycyclohexanone, D,L-2,3,4,6/5-pentacyclohexanone and 2,2'-anhydro-2-C-hydroxymethyl-myo-inositol, but at lower rates than that of myo-inositol.
  • Formerly EC 2.1.1.134.
 2 P45986 P45986
Tetrahydrocolumbamine 2-O-methyltransferase. [EC: 2.1.1.89]
S-adenosyl-L-methionine + 5,8,13,13a-tetrahydrocolumbamine = S-adenosyl- L-homocysteine + tetrahydropalmatine.
  • Involved in the biosynthesis of the berberine alkaloids.
 2 Q8H9A8 Q8H9A8
2,4,7-trihydroxy-1,4-benzoxazin-3-one-glucoside 7-O-methyltransferase. [EC: 2.1.1.241]
S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4- benzoxazin-2-yl beta-D-glucopyranoside = S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside.
  • The enzyme is involved in the biosynthesis of the protective and allelophatic benzoxazinoid DIMBOA ((2R)-4-hydroxy-7-methoxy-3-oxo- 3,4-dihydro-2H-1,4-benzoxazin) in some plants, most commonly from the family of Poacea (grasses).
 2 B1P123 B1P123
Isoliquiritigenin 2'-O-methyltransferase. [EC: 2.1.1.154]
S-adenosyl-L-methionine + isoliquiritigenin = S-adenosyl-L-homocysteine + 2'-O-methylisoliquiritigenin.
  • Not identical to EC 2.1.1.65.
  • While EC 2.1.1.154 can use licodione as a substrate, EC 2.1.1.65 cannot use isoliquiritigenin as a substrate.
 2 P93324 P93324
Flavonoid 3',5'-methyltransferase. [EC: 2.1.1.267]
(1) S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L- homocysteine + a 3'-methoxyflavonoid. (2) S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid.
  • Isolated from Vitis vinifera (grape).
  • Most active with delphinidin 3-glucoside but also acts on cyanidin 3-glucoside, cyanidin, myricetin, quercetin and quercetin 3-glucoside.
  • The enzyme from Catharanthus roseus was most active with myricetin.
  • Formerly EC 2.1.1.149.
 2 Q8GSN1 Q8GSN1
Trans-anol O-methyltransferase. [EC: 2.1.1.279]
(1) S-adenosyl-L-methionine + trans-anol = S-adenosyl-L-homocysteine + trans-anethole. (2) S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol.
  • The enzyme from anise (Pimpinella anisum) is highly specific for substrates in which the double bond in the propenyl side chain is located between C(7) and C(8), and, in contrast to EC 2.1.1.146, does not have activity with eugenol or chavicol.
 2 B8RCD3 B8RCD3
(R,S)-reticuline 7-O-methyltransferase. [EC: 2.1.1.291]
(1) S-adenosyl-L-methionine + (S)-reticuline = S-adenosyl-L-homocysteine + (S)-laudanine. (2) S-adenosyl-L-methionine + (R)-reticuline = S-adenosyl-L-homocysteine + (R)-laudanine.
  • The enzyme from the plant Papaver somniferum (opium poppy) methylates (S)- and (R)-reticuline with equal efficiency and is involved in the biosynthesis of tetrahydrobenzylisoquinoline alkaloids.
 2 Q6WUC2 Q6WUC2
8-hydroxyquercetin 8-O-methyltransferase. [EC: 2.1.1.88]
S-adenosyl-L-methionine + 3,3',4',5,7,8-hexahydroxyflavone = S-adenosyl- L-homocysteine + 3,3',4',5,7-pentahydroxy-8-methoxyflavone.
  • Also acts on 8-hydroxykaempferol, but not on the glycosides of 8-hydroxyflavonols.
  • An enzyme from the flower buds of Lotus corniculatus.
 2 Q6VMW0 Q6VMW0
Flavonoid 4'-O-methyltransferase. [EC: 2.1.1.231]
S-adenosyl-L-methionine + a 4'-hydroxyflavanone = S-adenosyl-L- homocysteine + a 4'-methoxyflavanone.
  • The enzyme catalyzes the 4'-methylation of naringenin.
  • In vitro it catalyzes the 4'-methylation of apigenin, quercetin, daidzein and genistein.
 2 C6TAY1 C6TAY1
Mitomycin 6-O-methyltransferase. [EC: 2.1.1.316]
(1) S-adenosyl-L-methionine + 6-demethylmitomycin A = S-adenosyl-L- homocysteine + mitomycin A. (2) S-adenosyl-L-methionine + 6-demethylmitomycin B = S-adenosyl-L- homocysteine + mitomycin B.
  • The enzyme, characterized from the bacterium Streptomyces lavendulae, is involved in the biosynthesis of the quinone-containing antibiotics mitomycin A and mitomycin B.
 2 A0A1K2FHL7 A0A1K2FHL7
Trans-resveratrol di-O-methyltransferase. [EC: 2.1.1.240]
2 S-adenosyl-L-methionine + trans-resveratrol = 2 S-adenosyl-L- homocysteine + pterostilbene.
  • The enzyme catalyzes the biosynthesis of pterostilbene from resveratrol.
 2 B6VJS4 B6VJS4
Columbamine O-methyltransferase. [EC: 2.1.1.118]
S-adenosyl-L-methionine + columbamine = S-adenosyl-L-homocysteine + palmatine.
  • The product of this reaction is a protoberberine alkaloid that is widely distributed in the plant kingdom.
  • Distinct in specificity from EC 2.1.1.88.
 2 Q8H9A8 Q8H9A8
Demethylspheroidene O-methyltransferase. [EC: 2.1.1.210]
S-adenosyl-L-methionine + demethylspheroidene = S-adenosyl-L-homocysteine + spheroidene.
  • In Rhodopseudomonas capsulata and Rubrivivax gelatinosus the enzyme is involved in biosynthesis of spheroidene.
  • In Rubrivivax gelatinosus the enzyme also catalyzes the methylation of demethylspirilloxanthin to spirilloxanthin and the methylation of 3,4-didehydrorhodopin to anhydrorhodovibrin.
 2 A0A1A0CZE4 A0A1A0CZE4
Licodione 2'-O-methyltransferase. [EC: 2.1.1.65]
S-adenosyl-L-methionine + licodione = S-adenosyl-L-homocysteine + 2'-O- methyllicodione.
  • As well as licodione (1-(2,4-dihydroxyphenyl)-3-(4-hydroxyphenyl)- 1,3-propanedione), the 2''-hydroxy-derivative and isoliquiritigenin can act as acceptors, more slowly.
 2 P93324 P93324
Tricetin 3',4',5'-O-trimethyltransferase. [EC: 2.1.1.169]
3 S-adenosyl-L-methionine + tricetin = 3 S-adenosyl-L-homocysteine + 3',4',5'-O-trimethyltricetin.
  • The enzyme from Triticum aestivum catalyzes the sequential O-methylation of tricetin via 3'-O-methyltricetin, 3',5'-O- methyltricetin to 3',4',5'-O-trimethyltricetin (2).
 2 Q38J50 Q38J50
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