CATH Classification

Domain Context

CATH Clusters

Superfamily P-loop containing nucleotide triphosphate hydrolases
Functional Family CTP synthase, putative, expressed

Enzyme Information

6.3.4.2
CTP synthase (glutamine hydrolyzing).
based on mapping to UniProt P17812
ATP + UTP + L-glutamine = ADP + phosphate + CTP + L-glutamate.
-!- The enzyme contains three functionally distinct sites: an allosteric GTP-binding site, a glutaminase site where glutamine hydrolysis occurs (cf. EC 3.5.1.2), and the active site where CTP synthesis takes place. -!- The reaction proceeds via phosphorylation of UTP by ATP to give an activated intermediate 4-phosphoryl UTP and ADP. -!- Ammonia then reacts with this intermediate generating CTP and a phosphate. -!- The enzyme can also use ammonia from the surrounding solution.

UniProtKB Entries (1)

P17812
PYRG1_HUMAN
Homo sapiens
CTP synthase 1

PDB Structure

PDB 2VO1
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of the Synthetase Domain of Human Ctp Synthetase, a Target for Anticancer Therapy.
Kursula, P., Flodin, S., Ehn, M., Hammarstrom, M., Schuler, H., Nordlund, P., Stenmark, P.
Acta Crystallogr.,Sect.F