CATH Classification

Domain Context

CATH Clusters

Superfamily Aldolase class I
Functional Family Glutamate synthase, large subunit

Enzyme Information

1.4.1.13
Glutamate synthase (NADPH).
based on mapping to UniProt Q05755
2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH.
-!- The reaction takes place in the opposite direction. -!- The protein is composed of two subunits, alpha and beta. -!- The alpha subunit is composed of two domains, one hydrolyzing L-glutamine to NH(3) and L-glutamate (cf. EC 3.5.1.2), the other combining the produced NH(3) with 2-oxoglutarate to produce a second molecule of L-glutamate (cf. EC 1.4.1.4). -!- The beta subunit transfers electrons to the cosubstrate. -!- The NH(3) is channeled through a 31 A channel in the active protein. -!- In the absence of the beta subunit, coupling between the two domains of the alpha subunit is compromised and some ammonium can be produced. -!- In the intact alpha-beta complex, ammonia production only takes place as part of the overall reaction. -!- Formerly EC 2.6.1.53.

UniProtKB Entries (1)

Q05755
GLTB_AZOBR
Azospirillum brasilense
Glutamate synthase [NADPH] large chain

PDB Structure

PDB 1EA0
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Cross-Talk and Ammonia Channeling between Active Centers in the Unexpected Domain Arrangement of Glutamate Synthase
Binda, C., Bossi, R.T., Wakatsuki, S., Arzt, S., Coda, A., Curti, B., Vanoni, M.A., Mattevi, A.
Structure