CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.1280 | Alpha/beta knot |
|
3.40.1280.10 | SPOUT methyltransferase, trefoil knot domain |
Domain Context
CATH Clusters
| Superfamily | 3.40.1280.10 |
| Functional Family | Ribosomal RNA small subunit methyltransferase |
Enzyme Information
| 2.1.1.260 |
rRNA small subunit pseudouridine methyltransferase Nep1.
based on mapping to UniProt Q06287
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA.
-!- This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. -!- It recognizes Psi(914) in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi(1191) in yeast 18S rRNA, and Psi(1248) in human 18S rRNA.
|
UniProtKB Entries (1)
| Q06287 |
NEP1_YEAST
Saccharomyces cerevisiae S288C
Ribosomal RNA small subunit methyltransferase NEP1
|
PDB Structure
| PDB | 3OIJ |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis.
Nucleic Acids Res.
|
