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CATH Superfamily 3.90.1200.10

Aminoglycoside phosphotransferase (APH), C-terminal lobe

The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was: waiting to be named.

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.

Superfamily EC Annotations

Note: the EC figure is not being displayed for this superfamily as there are more than 100 different EC terms.

There are 17 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
Thiamine kinase. [EC: 2.7.1.89]
ATP + thiamine = ADP + thiamine phosphate.
    		 192 A0A026V8B6 A0A029I593 A0A029IX08 A0A037YCE0 A0A066SWV4 A0A069XL01 A0A070SPY9 A0A070V142 A0A073HYB9 A0A074I7F1
    (182 more...)
    Kanamycin kinase. [EC: 2.7.1.95]
    ATP + kanamycin = ADP + kanamycin 3'-phosphate.
    • Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A.
    • An enzyme from Pseudomonas aeruginosa also acts on butirosin.
    		 172 A0A059N2T2 A0A062FBM4 A0A068DRS3 A0A078RD37 A0A084SQA4 A0A088F9G6 A0A0C3I4F5 A0A0D5C7M3 A0A0E1AK15 A0A0K2CTV4
    (162 more...)
    Homoserine kinase. [EC: 2.7.1.39]
    ATP + L-homoserine = ADP + O-phospho-L-homoserine.
      		 156 A0A059FZR6 A0A066PVC0 A0A069BES1 A0A069PXS0 A0A083ZQ51 A0A095F6W4 A0A0A8F5I6 A0A0C6ETU7 A0A0E0UAE4 A0A0E1VWL3
      (146 more...)
      Aminoglycoside 2''-phosphotransferase. [EC: 2.7.1.190]
      GTP + gentamicin = GDP + gentamicin 2''-phosphate.
      • This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin.
      • In most, but not all, cases the phosphorylation confers resistance against the antibiotic.
      • Some forms of the enzyme use ATP as a phosphate donor in appreciable amount.
      • The enzyme is often found as a bifunctional enzyme that also catalyzes 6'-aminoglycoside N-acetyltransferase activity.
      • The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.
      		 125 A0A059N3E0 A0A0M1X7S9 A0A0U1B434 A0A125W300 A0A141BHX6 A0A193AUS1 A0A1B1UXP7 A0A1C6GZY0 A0A1S1ZSS5 A0A1X9I6C2
      (115 more...)
      S-methyl-5-thioribose kinase. [EC: 2.7.1.100]
      ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate.
      • Also acts, more slowly, on CTP.
      		 104 A0A0B5XG06 A0A0B5XYW3 A0A0E1CI25 A0A0G8E9G8 A0A0H3GPU2 A0A0J7GLC3 A0A0Q0ZGK7 A0A0T8FVR7 A0A0T8XN83 A0A0U1EPC7
      (94 more...)
      Maltokinase. [EC: 2.7.1.175]
      ATP + maltose = ADP + alpha-maltose 1-phosphate.
      • Formerly EC 2.7.1.n8.
      		 32 A0A045I1G1 A0A049DUT9 A0A0H3L4N9 A0A0K2HR26 A0A0T9N5U0 A0A109SJW2 A0A109ST12 A0A117DTZ5 A0A1X0GTY1 A0A328H128
      (22 more...)
      Ethanolamine kinase. [EC: 2.7.1.82]
      ATP + ethanolamine = ADP + O-phosphoethanolamine.
        		 25 A0A024R4X4 A0A024R9A8 A7MCT6 A7SK27 D3ZRW8 G2WAP7 K7D959 N1P6E5 O54783 O54804
        (15 more...)
        Hygromycin B 4-O-kinase. [EC: 2.7.1.163]
        ATP + hygromycin B = ADP + 4-O-phosphohygromycin B.
        • Phosphorylates the antibiotic hygromycin B.
        • Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B.
        • Formerly EC 2.7.1.n6.
        		 19 A0A097J0L4 A0A127GU86 A0A172QY28 A0A1C9T7Y7 A0A218MNY4 A0A2T4CKX8 A0A3R0BMP0 A0A3S7EAN5 A0A3T2ZWP2 A0A3T3DCN5
        (9 more...)
        Choline kinase. [EC: 2.7.1.32]
        ATP + choline = ADP + phosphocholine.
        • Ethanolamine and its methyl and ethyl derivatives can also act as acceptors.
        		 18 A0A024R4X4 N1NY98 O54783 O54804 O55229 P20485 P35790 P46558 P46559 P46560
        (8 more...)
        N-acetylmuramate 1-kinase. [EC: 2.7.1.221]
        ATP + N-acetyl-D-muramate = ADP + N-acetyl-alpha-D-muramate 1-phosphate.
        • The enzyme, characterized from Pseudomonas species, participates in a peptidoglycan salvage pathway.
        		 8 A0A0H3CDX5 A0A2V4FAN7 A0A454M1Y7 E6MWG9 Q88QT3 Q9A2M2 Q9I5U1 Q9K188
        Hydroxylysine kinase. [EC: 2.7.1.81]
        GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine.
        • Both the natural 5-hydroxy-L-lysine and its 5-epimer act as acceptors.
        		 6 A2RU49 A5PJU6 A8WFT6 B7ZVI3 Q5U5V2 Q6PB06
        Protein-ribulosamine 3-kinase. [EC: 2.7.1.172]
        ATP + [protein]-N(6)-D-ribulosyl-L-lysine = ADP + [protein]-N(6)-(3-O- phospho-D-ribulosyl)-L-lysine.
        • This enzyme plays a role in freeing proteins from ribulosamines or psicosamines, which might arise from the reaction of amines with glucose and/or glycolytic intermediates.
        • This role is shared by EC 2.7.1.171, which has, in addition, the unique capacity to phosphorylate fructosamines.
        • The plant enzyme also phosphorylates [protein]-N(6)-D-erythrulosyl-L- lysine.
        • No activity with [protein]-N(6)-D-fructosyl-L-lysine.
        		 5 A0A0E0IK39 A0A0E0NNU5 A2XBT1 Q10SM2 Q9LEW8
        4-hydroxytryptamine kinase. [EC: 2.7.1.222]
        ATP + 4-hydroxytryptamine = ADP + 4-hydoxytryptamine 4-phosphate.
        • Also acts on 4-hydroxy-L-tryptophan in vitro.
        • Isolated from the fungus Psilocybe cubensis.
        • Involved in the biosynthesis of the psychoactive compound psilocybin.
        		 2 A0A286LEZ6 P0DPA8
        N-acetylhexosamine 1-kinase. [EC: 2.7.1.162]
        ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate.
        • Involved in the lacto-N-biose I/galacto-N-biose degradation pathway in the probiotic bacterium Bifidobacterium longum.
        • Differs from EC 2.7.1.157 as it can phosphorylate both N-acetylgalactosamine and N-acetylglucosamine at similar rates.
        • Also has some activity with N-acetyl-D-mannosamine, D-talose and D-mannose as substrate.
        • ATP can be replaced by GTP or ITP but with decreased enzyme activity.
        		 2 A0A413AI77 E8MF12
        Viomycin kinase. [EC: 2.7.1.103]
        ATP + viomycin = ADP + O-phosphoviomycin.
        • Also acts on capreomycins.
        • A serine residue in the peptide antibiotics acts as phosphate- acceptor.
        		 1 P18623
        Hygromycin-B 7''-O-kinase. [EC: 2.7.1.119]
        ATP + hygromycin B = ADP + 7''-O-phosphohygromycin B.
        • Phosphorylates the antibiotics hygromycin B, 1-N-hygromycin B and destomycin, but not hygromycin B2, at the 7''-hydroxy group in the destomic acid ring.
        		 1 P09979
        Streptomycin 3''-kinase. [EC: 2.7.1.87]
        ATP + streptomycin = ADP + streptomycin 3''-phosphate.
        • Also phosphorylates dihydrostreptomycin, 3'-deoxydihydrostreptomycin and their 6-phosphates.
        		 1 P18150