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CATH Superfamily 3.40.910.10

Deoxyhypusine synthase

The name of this superfamily has been modified since the most recent official CATH+ release (v4_3_0). At the point of the last release, this superfamily was named:

"
Deoxyhypusine synthase
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 16: Deoxyhypusine synthase

There are 1 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
Deoxyhypusine synthase. [EC: 2.5.1.46]
[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.
  • The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity.
  • Catalyzes the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor.
  • The reaction occurs in four steps: NAD(+)-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d).
  • Hence the overall reaction is transfer of a 4-aminobutyl group.
  • For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor.
  • Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29.
  • Formerly EC 1.1.1.249.
 1 B5APK2