CATH Domain 6isuA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.532	Ubiquitin C-terminal Hydrolase UCH-l3
	3.40.532.10	Peptidase C12, ubiquitin carboxyl-terminal hydrolase

Domain Context

CATH Clusters

Superfamily	Peptidase C12, ubiquitin carboxyl-terminal hydrolase
Functional Family	Ubiquitin carboxyl-terminal hydrolase

Enzyme Information

3.4.19.12

Ubiquitinyl hydrolase 1.

based on mapping to UniProt P15374

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P0CG47

UBB_HUMAN

Homo sapiens

Polyubiquitin-B

PDB Structure

PDB	6ISU
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Chemical Protein Synthesis Enabled Mechanistic Studies on the Molecular Recognition of K27-linked Ubiquitin Chains. Pan, M., Zheng, Q., Ding, S., Zhang, L., Qu, Q., Wang, T., Hong, D., Ren, Y., Liang, L., Chen, C., Mei, Z., Liu, L. Angew. Chem. Int. Ed. Engl.