CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family

Enzyme Information

2.1.1.226
23S rRNA (cytidine(1920)-2'-O)-methyltransferase.
based on mapping to UniProt P9WJ63
S-adenosyl-L-methionine + cytidine(1920) in 23S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1920) in 23S rRNA.
-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1920) in helix 69 of 23S rRNA and cytidine(1409) in helix 44 of 16S rRNA (cf. EC 2.1.1.227). -!- These methylations result in increased susceptibility to the antibiotics capreomycin and viomycin.
2.1.1.227
16S rRNA (cytidine(1409)-2'-O)-methyltransferase.
based on mapping to UniProt P9WJ63
S-adenosyl-L-methionine + cytidine(1409) in 16S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1409) in 16S rRNA.
-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1409) in helix 44 of 16S rRNA and cytidine(1920) in helix 69 of 23S rRNA (cf. EC 2.1.1.226).

UniProtKB Entries (1)

P9WJ63
TLYA_MYCTU
Mycobacterium tuberculosis H37Rv
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA

PDB Structure

PDB 5KS2
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
A Novel Motif for S-Adenosyl-l-methionine Binding by the Ribosomal RNA Methyltransferase TlyA from Mycobacterium tuberculosis.
Witek, M.A., Kuiper, E.G., Minten, E., Crispell, E.K., Conn, G.L.
J. Biol. Chem.