CATH Classification

Domain Context

CATH Clusters

Superfamily Rieske [2Fe-2S] iron-sulphur domain
Functional Family 3-ketosteroid-9-alpha-hydroxylase oxygenase subunit

Enzyme Information

1.14.15.30
3-ketosteroid 9-alpha-monooxygenase.
based on mapping to UniProt P71875
Androsta-1,4-diene-3,17-dione + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = 9-alpha-hydroxyandrosta-1,4-diene-3,17-dione + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O.
-!- The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. -!- It forms a two-component system with a ferredoxin reductase (KshB). -!- The enzyme contains a Rieske-type iron-sulfur center and non-heme iron. -!- The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione. -!- Formerly EC 1.14.13.142.

UniProtKB Entries (1)

P71875
KSHA_MYCTU
Mycobacterium tuberculosis H37Rv
3-ketosteroid-9-alpha-monooxygenase, oxygenase component

PDB Structure

PDB 4QCK
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Substrate specificities and conformational flexibility of 3-ketosteroid 9 alpha-hydroxylases.
Penfield, J.S., Worrall, L.J., Strynadka, N.C., Eltis, L.D.
J.Biol.Chem.