CATH Classification

Domain Context

CATH Clusters

Superfamily Chondroitin AC/alginate lyase
Functional Family Alginate lyase

Enzyme Information

4.2.2.3
Mannuronate-specific alginate lyase.
based on mapping to UniProt Q06749
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy- alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
-!- The enzyme catalyzes the degradation of alginate by a beta- elimination reaction. -!- It cleaves the (1->4) bond between beta-D-mannuronate and either alpha-L-guluronate or beta-D-mannuronate, generating oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non- reducing ends and beta-D-mannuronate at the reducing end. -!- Depending on the composition of the substrate, the enzyme produces oligosaccharides ranging from two to four residues, with preference for shorter products. -!- Cf. EC 4.2.2.11. -!- Formerly EC 4.2.99.4.

UniProtKB Entries (1)

Q06749
ALGL_PSEAE
Pseudomonas aeruginosa PAO1
Alginate lyase

PDB Structure

PDB 4OZW
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal Structure of the periplasmic alginate lyase AlgL H202A mutant
Howell, P.L., Wolfram, F., Robinson, H., Arora, K.
To be published