CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.47.10
Functional Family 3-ketoacyl-CoA thiolase, mitochondrial

Enzyme Information

2.3.1.16
Acetyl-CoA C-acyltransferase.
based on mapping to UniProt P42765
Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
-!- The enzyme, found in both eukaryotes and in prokaryotes, is involved in degradation pathways such as fatty acid beta-oxidation. -!- The enzyme acts on 3-oxoacyl-CoAs to produce acetyl-CoA and an acyl- CoA shortened by two carbon atoms. -!- The reaction starts with the acylation of a nucleophilic cysteine at the active site by a 3-oxoacyl-CoA, with the concomitant release of acetyl-CoA. -!- In the second step the acyl group is transferred to CoA. -!- Most enzymes have a broad substrate range for the 3-oxoacyl-CoA. -!- cf. EC 2.3.1.9.

UniProtKB Entries (1)

P42765
THIM_HUMAN
Homo sapiens
3-ketoacyl-CoA thiolase, mitochondrial

PDB Structure

PDB 4C2K
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The Crystal Structure of Human Mitochondrial 3-Ketoacyl-Coa Thiolase (T1): Insight Into the Reaction Mechanism of its Thiolase and Thioesterase Activities
Kiema, T.-R., Harijan, R.K., Strozyk, M., Fukao, T., Alexson, S.E.H., Wierenga, R.K.
Acta Crystallogr.,Sect.D