CATH Domain 4altA00

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.70	Distorted Sandwich
	2.70.50	Coagulation Factor XIII; Chain A, domain 1
	2.70.50.50	chitin-binding protein cbp21

Domain Context

CATH Clusters

Superfamily	chitin-binding protein cbp21
Functional Family	Chitin binding domain protein

Enzyme Information

1.14.99.53

Lytic chitin monooxygenase.

based on mapping to UniProt Q838S1

((1->4)-N-acetyl-beta-D-glucosaminyl)(n+m) + reduced acceptor + O(2) = ((1->4)-N-acetyl-beta-D-glucosaminyl)(m-1)-(1->4)-2-(acetylamino)-2- deoxy-D-glucono-1,5-lactone + ((1->4)-N-acetyl-beta-D-glucosaminyl)(n) + acceptor + H(2)O.

-!- The enzyme cleaves chitin in an oxidative manner, releasing fragments of chitin with an N-acetylamino-D-glucono-1,5-lactone at the reducing end. -!- The initially formed lactone at the reducing end of the shortend chitin chain quickly hydrolyzes spontaneously to the aldonic acid. -!- In vitro ascorbate can serve as reducing agent.

UniProtKB Entries (1)

Q838S1

LCHMO_ENTFA

Enterococcus faecalis V583

Lytic chitin monooxygenase

PDB Structure

PDB	4ALT
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural and Electronic Snapshots During the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-Ray Photo-Reduction. Gudmundsson, M., Kim, S., Wu, M., Ishida, T., Haddad Momeni, M., Vaaje-Kolstad, G., Lundberg, D., Royant, A., Stahlberg, J., Eijsink, V.G., Beckham, G.T., Sandgren, M. J.Biol.Chem.