CATH Classification

Domain Context

CATH Clusters

Superfamily Ribonuclease E inhibitor RraA/RraA-like
Functional Family 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase

Enzyme Information

4.1.3.17
4-hydroxy-4-methyl-2-oxoglutarate aldolase.
based on mapping to UniProt A5W059
(1) 4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate. (2) 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate.
-!- This enzyme participates in the degradation of protocatechuate (via the meta-cleavage pathway), phthalate, syringate and gallate. -!- The enzyme from Pseudomonas ochraceae can also cleave 4-hydroxy-2- oxoglutarate to glyoxylate and pyruvate, and also catalyzes the reaction of EC 4.1.1.3.
4.1.1.112
Oxaloacetate decarboxylase.
based on mapping to UniProt A5W059
Oxaloacetate = pyruvate + CO(2).
-!- The enzymes from the fish Gadus morhua (Atlantic cod) and the bacterium Micrococcus luteus prefer Mn(2+), while those from the bacteria Pseudomonas putida and Pseudomonas aeruginosa prefer Mg(2+). -!- Unlike EC 7.2.4.2, there is no evidence of the enzyme's involvement in Na(+) transport. -!- Formerly EC 4.1.1.3.

UniProtKB Entries (1)

A5W059
HMGA_PSEP1
Pseudomonas putida F1
4-hydroxy-4-methyl-2-oxoglutarate aldolase/4-carboxy-4-hydroxy-2-oxoadipate aldolase

PDB Structure

PDB 3NOJ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural and Kinetic Characterization of 4-Hydroxy-4-methyl-2-oxoglutarate/4-Carboxy-4-hydroxy-2-oxoadipate Aldolase, a Protocatechuate Degradation Enzyme Evolutionarily Convergent with the HpaI and DmpG Pyruvate Aldolases.
Wang, W., Mazurkewich, S., Kimber, M.S., Seah, S.Y.
J.Biol.Chem.