CATH Domain 3kv4A01
|Functional Family||histone lysine demethylase PHF8 isoform X1|
[Histone H3]-dimetyl-L-lysine-36 demethylase.
based on mapping to UniProt Q9UPP1
[Protein]-N(6),N(6)-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O(2) = [protein]-L-lysine + 2 succinate + 2 formaldehyde + 2 CO(2).
-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.
UniProtKB Entries (1)
Histone lysine demethylase PHF8
Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases.