CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family Steroid C26-monooxygenase

Enzyme Information

1.14.15.29
Cholest-4-en-3-one 26-monooxygenase ((25S)-3-oxocholest-4-en-26-oate forming).
based on mapping to UniProt P9WPP1
Cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H(+) + 3 O(2) = (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron- sulfur] cluster + 4 H(2)O.
-!- A cytochrome P450 (heme-thiolate) protein found in several bacterial pathogens. -!- The enzyme is involved in degradation of the host's cholesterol. -!- It catalyzes the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol. -!- The products are exclusively in the (25S) conformation. -!- The enzyme also accepts cholesterol as a substrate. -!- Cf. EC 1.14.15.28. -!- Formerly EC 1.14.13.141.

UniProtKB Entries (1)

P9WPP1
CP125_MYCTU
Mycobacterium tuberculosis H37Rv
Steroid C26-monooxygenase

PDB Structure

PDB 3IW2
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection.
McLean, K.J., Lafite, P., Levy, C., Cheesman, M.R., Mast, N., Pikuleva, I.A., Leys, D., Munro, A.W.
J.Biol.Chem.