CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family Epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthase

Enzyme Information

4.2.3.47
Beta-farnesene synthase.
based on mapping to UniProt Q9K498
(2E,6E)-farnesyl diphosphate = (E)-beta-farnesene + diphosphate.
1.14.13.106
Epi-isozizaene 5-monooxygenase.
based on mapping to UniProt Q9K498
(+)-epi-isozizaene + 2 NADPH + 2 O(2) = albaflavenone + 2 NADP(+) + 3 H(2)O.
-!- This enzyme, from the soil-dwelling bacterium Streptomyces coelicolor A3(2), catalyzes two sequential allylic oxidation reactions. -!- The substrate epi-isozizaene, which is formed by the action of EC 4.2.3.37 is first oxidized to yield the epimeric intermediates (5R)-albaflavenol and (5S)-albaflavenol, which can be further oxidized to yield the sesquiterpenoid antibiotic albaflavenone.

UniProtKB Entries (1)

Q9K498
EIZFM_STRCO
Streptomyces coelicolor A3(2)
Epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthase

PDB Structure

PDB 3EL3
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structure of albaflavenone monooxygenase containing a moonlighting terpene synthase active site
Zhao, B., Lei, L., Vassylyev, D.G., Lin, X., Cane, D.E., Kelly, S.L., Yuan, H., Lamb, D.C., Waterman, M.R.
J.Biol.Chem.