CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family Epoxide hydrolase B

Enzyme Information

3.3.2.10
Soluble epoxide hydrolase.
based on mapping to UniProt P95276
An epoxide + H(2)O = a glycol.
-!- Catalyzes the hydrolysis of trans-substituted epoxides, such as trans-stilbene oxide, as well as various aliphatic epoxides derived from fatty-acid metabolism. -!- It is involved in the metabolism of arachidonic epoxides (epoxyeicosatrienoic acids; EETs) and linoleic acid epoxides. -!- The enzyme from mammals is a bifunctional enzyme: the C-terminal domain exhibits epoxide-hydrolase activity and the N-terminal domain has the activity of EC 3.1.3.76. -!- Like EC 3.3.2.9, it is probable that the reaction involves the formation of an hydroxyalkyl-enzyme intermediate. -!- The enzyme can also use leukotriene A(4), the substrate of EC 3.3.2.6, but it forms 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than leukotriene B(4) as the product. -!- Formerly EC 3.3.2.3, EC 4.2.1.63 and EC 4.2.1.64.

UniProtKB Entries (1)

P95276
EPHB_MYCTO
Mycobacterium tuberculosis CDC1551
Epoxide hydrolase B

PDB Structure

PDB 2ZJF
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The molecular structure of epoxide hydrolase B from Mycobacterium tuberculosis and its complex with a urea-based inhibitor.
Biswal, B.K., Morisseau, C., Garen, G., Cherney, M.M., Garen, C., Niu, C., Hammock, B.D., James, M.N.
J.Mol.Biol.