CATH Classification

Domain Context

CATH Clusters

Superfamily 1.10.720.60
Functional Family Enolase-phosphatase E1

Enzyme Information

3.1.3.77
Acireductone synthase.
based on mapping to UniProt P32626
5-(methylthio)-2,3-dioxopentyl phosphate + H(2)O = 1,2-dihydroxy-5- (methylthio)pent-1-en-3-one + phosphate.
-!- This bifunctional enzyme first enolizes the substrate to form the intermediate 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2- dihydroxy-5-(methylsulfanyl)pent-1-en-3-one. -!- The acireductone represents a branch point in the methione salvage pathway as it is used in the formation of formate, CO and 3-(methylsulfanyl)propanoate by EC 1.13.11.53 and of formate and 4-(methylsulfanyl)-2-oxobutanoate either by a spontaneous reaction under aerobic conditions or by EC 1.13.11.54.

UniProtKB Entries (1)

P32626
ENOPH_YEAST
Saccharomyces cerevisiae S288C
Enolase-phosphatase E1

PDB Structure

PDB 2G80
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of UTR4 protein (Unknown transcript 4 protein) (yel038w) from Saccharomyces cerevisiae at 2.28 A resolution
Joint Center for Structural Genomics (JCSG)
To be published