CATH Classification

Domain Context

CATH Clusters

Superfamily Mannitol-specific EII; Chain A
Functional Family PTS system mannitol-specific transporter subunit IICBA

Enzyme Information

2.7.1.197
Protein-N(pi)-phosphohistidine--D-mannitol phosphotransferase.
based on mapping to UniProt P00550
[Protein]-N(pi)-phospho-L-histidine + D-mannitol(Side 1) = [protein]-L- histidine + D-mannitol 1-phosphate(Side 2).
-!- This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). -!- The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. -!- The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9 (phosphoenolpyruvate--protein phosphotransferase). -!- Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. -!- The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate. -!- Formerly EC 2.7.1.69.

UniProtKB Entries (1)

P00550
PTM3C_ECOLI
Escherichia coli K-12
PTS system mannitol-specific EIICBA component

PDB Structure

PDB 2FEW
External Links
Method SOLUTION NMR
Organism Escherichia
Primary Citation
Solution Structure of a Post-transition State Analog of the Phosphotransfer Reaction between the A and B Cytoplasmic Domains of the Mannitol Transporter IIMannitol of the Escherichia coli Phosphotransferase System
Suh, J.Y., Cai, M., Williams Jr., D.C., Clore, G.M.
J.Biol.Chem.