CATH Classification

Domain Context

CATH Clusters

Superfamily Bira Bifunctional Protein; Domain 2
Functional Family O-phosphoserine--tRNA(Cys) ligase

Enzyme Information

6.1.1.27
O-phosphoserine--tRNA ligase.
based on mapping to UniProt Q59054
ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L- seryl-tRNA(Cys).
-!- In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 for the direct Cys-tRNA(Cys) formation, Cys-tRNA(Cys) is produced by an indirect pathway, in which EC 6.1.1.27 ligates O-phosphoserine to tRNA(Cys), and EC 2.5.1.73 converts the produced O-phospho-L-seryl- tRNA(Cys) to Cys-tRNA(Cys). -!- The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism. -!- Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 and the indirect pathway with EC 6.1.1.27 and EC 2.5.1.73. -!- Formerly EC 6.1.1.n2.

UniProtKB Entries (1)

Q59054
SEPS_METJA
Methanocaldococcus jannaschii DSM 2661
O-phosphoserine--tRNA(Cys) ligase

PDB Structure

PDB 2DU7
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.
Fukunaga, R., Yokoyama, S.
Nat.Struct.Mol.Biol.