CATH Classification

Domain Context

CATH Clusters

Superfamily FAD/NAD(P)-binding domain
Functional Family protein-methionine sulfoxide oxidase MICAL2 isoform X1

Enzyme Information

1.14.13.225
F-actin monooxygenase.
based on mapping to UniProt Q8VDP3
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine- (R)-S-oxide + NADP(+) + H(2)O.
-!- The enzyme, characterized from the fruit fly Drosophila melanogaster, is a multi-domain oxidoreductase that acts as an F-actin disassembly factor. -!- The enzyme selectively reduces two L-Met residues of F-actin, causing fragmentation of the filaments and preventing repolymerization. -!- Free methionine is not a substrate. -!- The reaction is stereospecific and generates the (R)-sulfoxide.

UniProtKB Entries (1)

Q8VDP3
MICA1_MOUSE
Mus musculus
[F-actin]-monooxygenase MICAL1

PDB Structure

PDB 2C4C
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
High-Resolution Structure of the Catalytic Region of Mical (Molecule Interacting with Casl), a Multidomain Flavoenzyme-Signaling Molecule.
Siebold, C., Berrow, N., Walter, T.S., Harlos, K., Owens, R.J., Stuart, D.I., Terman, J.R., Kolodkin, A.L., Pasterkamp, R.J., Jones, E.Y.
Proc.Natl.Acad.Sci.USA