CATH Classification

Domain Context

CATH Clusters

Superfamily Cysteine proteinases
Functional Family Ubiquitin carboxyl-terminal hydrolase 14

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P54578
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P54578
UBP14_HUMAN
Homo sapiens
Ubiquitin carboxyl-terminal hydrolase 14

PDB Structure

PDB 2AYN
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14.
Hu, M., Li, P., Song, L., Jeffrey, P.D., Chenova, T.A., Wilkinson, K.D., Cohen, R.E., Shi, Y.
Embo J.