CATH Classification

Domain Context

CATH Clusters

Superfamily Pseudouridine synthase
Functional Family tRNA pseudouridine synthase B

Enzyme Information

5.4.99.25
tRNA pseudouridine(55) synthase.
based on mapping to UniProt P60340
tRNA uridine(55) = tRNA pseudouridine(55).
-!- Pseudourine synthase TruB from Escherichia coli specifically modifies uridine(55) in tRNA molecules. -!- The bifunctional archaeal enzyme also catalyzes the pseudouridylation of uridine(54). -!- It is not known whether the enzyme from Escherichia coli can also act on position 54 in vitro, since this position is occupied in E.coli tRNAs by thymine.

UniProtKB Entries (1)

P60340
TRUB_ECOLI
Escherichia coli K-12
TRNA pseudouridine synthase B

PDB Structure

PDB 1ZL3
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain
Hoang, C., Hamilton, C.S., Mueller, E.G., Ferre-D'Amare, A.R.
Protein Sci.