CATH Domain 1wegA01
|1.10.1670||Endonuclease Iii, domain 2|
|1.10.1670.10||Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)|
|Superfamily||Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)|
|Functional Family||Adenine DNA glycosylase|
based on mapping to UniProt P17802
Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.
-!- The enzyme serves as a mismatch repair enzyme that works to correct 7,8-dihydro-8-oxoguanine:adenine mispairs that arise in DNA when error-prone synthesis occurs past 7,8-dihydro-8-oxoguanine (GO) lesions in DNA. -!- The enzyme excises the adenine of the mispair, producing an apurinic site sensitive to AP endonuclease activity. -!- After removing the undamaged adenine the enzyme remains bound to the site to prevent EC 188.8.131.52 (MutM) from removing the GO lesion, which could lead to a double strand break. -!- In vitro the enzyme is also active with adenine:guanine, adenine:cytosine, and adenine:7,8-dihydro-8-oxoadenine (AO) mispairs, removing the adenine in all cases.
UniProtKB Entries (1)
Escherichia coli K-12
Adenine DNA glycosylase
Reaction intermediates in the catalytic mechanism of Escherichia coli MutY DNA glycosylase