CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.160.20
Functional Family Ribonuclease 3

Enzyme Information

3.1.26.3
Ribonuclease III.
based on mapping to UniProt Q02555
Endonucleolytic cleavage to 5'-phosphomonoester.
-!- An endoribonuclease that cleaves double-stranded RNA molecules. -!- The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site. -!- Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage. -!- RNase III is involved in both rRNA processing and mRNA processing and decay.

UniProtKB Entries (1)

Q02555
RNT1_YEAST
Saccharomyces cerevisiae S288C
Ribonuclease 3

PDB Structure

PDB 1T4O
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse III
Leulliot, N., Quevillon-Cheruel, S., Graille, M., Van Tilbeurgh, H., Leeper, T.C., Godin, K.S., Edwards, T.E., Sigurdsson, S.T., Rozenkrants, N., Nagel, R.J., Ares, M., Varani, G.
Embo J.