CATH Classification

Domain Context

CATH Clusters

Superfamily Zn peptidases
Functional Family Aminoacylase 1

Enzyme Information

3.5.1.14
N-acyl-aliphatic-L-amino acid amidohydrolase.
based on mapping to UniProt Q03154
(1) An N-acyl-aliphatic-L-amino acid + H(2)O = an aliphatic L-amino acid + a carboxylate. (2) An N-acetyl-L-cysteine-S-conjugate + H(2)O = an L-cysteine-S- conjugate + acetate.
-!- The enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). -!- It acts on mercapturic acids (S-conjugates of N-acetyl-L-cysteine) and neutral aliphatic N-acyl-alpha-amino acids. -!- Some bacterial aminoacylases demonstrate substrate specificity of both EC 3.5.1.14 and EC 3.5.1.114. -!- Cf. EC 3.5.1.15 and EC 3.5.1.114.

UniProtKB Entries (1)

Q03154
ACY1_HUMAN
Homo sapiens
Aminoacylase-1

PDB Structure

PDB 1Q7L
External Links
Method X-RAY DIFFRACTION
Organism Spodoptera
Primary Citation
Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family.
Lindner, H.A., Lunin, V.V., Alary, A., Hecker, R., Cygler, M., Menard, R.
J.Biol.Chem.