CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.30 | 2-Layer Sandwich |
|
3.30.830 | Cytochrome Bc1 Complex; Chain A, domain 1 |
|
3.30.830.10 | Metalloenzyme, LuxS/M16 peptidase-like |
Domain Context
CATH Clusters
| Superfamily | Metalloenzyme, LuxS/M16 peptidase-like |
| Functional Family | Protease 3 |
Enzyme Information
| 3.4.24.55 |
Pitrilysin.
based on mapping to UniProt P05458
Preferential cleavage of 16-Tyr-|-Leu-17 and 25-Phe-|-Tyr-26 bonds of oxidized insulin B chain. Also acts on other substrates of Mw less than 7 kDa such as insulin and glucagon.
-!- From the periplasmic space of Escherichia coli. -!- Inhibited by EDTA and 1,10-phenanthroline; not thiol dependent. -!- Belongs to peptidase family M16. -!- Formerly EC 3.4.99.44.
|
UniProtKB Entries (1)
| P05458 |
PTRA_ECOLI
Escherichia coli K-12
Protease 3
|
PDB Structure
| PDB | 1Q2L |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Crystal Structure of pitrilysin, the prototype of insulin-degrading enzymes
To be Published
|