CATH Classification

Domain Context

CATH Clusters

Superfamily DHBP synthase
Functional Family 3,4-dihydroxy-2-butanone 4-phosphate synthase

Enzyme Information

4.1.99.12
3,4-dihydroxy-2-butanone-4-phosphate synthase.
based on mapping to UniProt Q60364
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.
-!- The reaction involves an intramolecular skeletal rearrangement, with the bonds in D-ribulose 5-phosphate that connect C-3 and C-5 to C-4 being broken, C-4 being removed as formate and reconnection of C-3 and C-5. -!- The phosphorylated four-carbon product (L-3,4-dihydroxybutan-2-one 4-phosphate) is an intermediate in the biosynthesis of riboflavin.

UniProtKB Entries (1)

Q60364
RIBB_METJA
Methanocaldococcus jannaschii DSM 2661
3,4-dihydroxy-2-butanone 4-phosphate synthase

PDB Structure

PDB 1PVW
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of 3,4-Dihydroxy-2-butanone 4-Phosphate Synthase from Methanococcus jannaschii in Complex with Divalent Metal Ions and the Substrate Ribulose 5-Phosphate: IMPLICATIONS FOR THE CATALYTIC MECHANISM
Steinbacher, S., Schiffmann, S., Richter, G., Huber, R., Bacher, A., Fischer, M.
J.Biol.Chem.