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CATH Superfamily 3.90.1200.10

Aminoglycoside phosphotransferase (APH), C-terminal lobe

The name of this superfamily has been modified since the most recent official CATH+ release (v4_2_0). At the point of the last release, this superfamily was: waiting to be named.

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 11127: Phosphotransferase enzyme family protein

There are 1 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
Aminoglycoside 2''-phosphotransferase. [EC: 2.7.1.190]
GTP + gentamicin = GDP + gentamicin 2''-phosphate.
  • This bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin.
  • In most, but not all, cases the phosphorylation confers resistance against the antibiotic.
  • Some forms of the enzyme use ATP as a phosphate donor in appreciable amount.
  • The enzyme is often found as a bifunctional enzyme that also catalyzes 6'-aminoglycoside N-acetyltransferase activity.
  • The bifunctional enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci.
 130 A0A059N3E0 A0A059N3E0 A0A069G178 A0A069G178 A0A0M1X7S9 A0A0M1X7S9 A0A0M1Z9N3 A0A0M1Z9N3 A0A0U1B434 A0A0U1B434
(120 more...)