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CATH Superfamily 2.40.50.140

Nucleic acid-binding proteins

The name of this superfamily has been modified since the most recent official CATH+ release (v4_2_0). At the point of the last release, this superfamily was named:

"
Nucleic acid-binding proteins
".

Functional Families

Overview of the Structural Clusters (SC) and Functional Families within this CATH Superfamily. Clusters with a representative structure are represented by a filled circle.
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FunFam 63197: Ribosomal protein S12 methylthiotransferase RimO

There are 1 EC terms in this cluster

Please note: EC annotations are assigned to the full protein sequence rather than individual protein domains. Since a given protein can contain multiple domains, it is possible that some of the annotations below come from additional domains that occur in the same protein, but have been classified elsewhere in CATH.

Note: The search results have been sorted with the annotations that are found most frequently at the top of the list. The results can be filtered by typing text into the search box at the top of the table.

EC Term Annotations Evidence
[Ribosomal protein S12] (aspartate(89)-C(3))-methylthiotransferase. [EC: 2.8.4.4]
L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.
  • This bacterial enzyme binds two [4Fe-4S] clusters.
  • A bridge of five sulfur atoms is formed between the free Fe atoms of the two [4Fe-4S] clusters.
  • In the first reaction the enzyme transfers a methyl group from AdoMet to the external sulfur ion of the sulfur bridge.
  • In the second reaction the enzyme catalyzes the reductive fragmentation of a second molecule of AdoMet, yielding a 5'-deoxyadenosine radical, which then attacks the methylated sulfur atom of the polysulfide bridge, resulting in the transfer of a methylthiol group to aspartate(89) (Escherichia coli numbering).
  • The enzyme is a member of the superfamily of S-adenosyl-L-methionine- dependent radical (radical AdoMet) enzymes.
 21 A0A085RGA9 A0A085TAB2 A0A085TJJ7 A0A0E3W471 A0A0H3Q428 A0A0H6BSY9 A0A0H7G2Y9 A0A0K9UP38 A0A0Q0TPK6 A0A0X1L449
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