CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.150 | Vaccinia Virus protein VP39 |
Domain Context
CATH Clusters
| Superfamily | Vaccinia Virus protein VP39 |
| Functional Family | Cytotoxin/hemolysin TlyA |
Enzyme Information
| 2.1.1.226 |
23S rRNA (cytidine(1920)-2'-O)-methyltransferase.
based on mapping to UniProt P9WJ63
S-adenosyl-L-methionine + cytidine(1920) in 23S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1920) in 23S rRNA.
-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1920) in helix 69 of 23S rRNA and cytidine(1409) in helix 44 of 16S rRNA (cf. EC 2.1.1.227). -!- These methylations result in increased susceptibility to the antibiotics capreomycin and viomycin.
|
| 2.1.1.227 |
16S rRNA (cytidine(1409)-2'-O)-methyltransferase.
based on mapping to UniProt P9WJ63
S-adenosyl-L-methionine + cytidine(1409) in 16S rRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(1409) in 16S rRNA.
-!- The bifunctional enzyme from Mycobacterium tuberculosis 2'-O- methylates cytidine(1409) in helix 44 of 16S rRNA and cytidine(1920) in helix 69 of 23S rRNA (cf. EC 2.1.1.226).
|
UniProtKB Entries (1)
| P9WJ63 |
TLYA_MYCTU
Mycobacterium tuberculosis H37Rv
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA
|
PDB Structure
| PDB | 5KS2 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
A Novel Motif for S-Adenosyl-l-methionine Binding by the Ribosomal RNA Methyltransferase TlyA from Mycobacterium tuberculosis.
J. Biol. Chem.
|