CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family Ribosomal RNA small subunit methyltransferase A

Enzyme Information

2.1.1.182
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase.
based on mapping to UniProt Q5SM60
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)- dimethyladenine(1519) in 16S rRNA.
-!- KsgA introduces the most highly conserved ribosomal RNA modification, the dimethylation of adenine(1518) and adenine(1519) in 16S rRNA. -!- Strains lacking the methylase are resistant to kasugamycin. -!- Formerly EC 2.1.1.48.

UniProtKB Entries (1)

Q5SM60
RSMA_THET8
Thermus thermophilus HB8
Ribosomal RNA small subunit methyltransferase A

PDB Structure

PDB 3FUU
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine.
Demirci, H., Belardinelli, R., Seri, E., Gregory, S.T., Gualerzi, C., Dahlberg, A.E., Jogl, G.
J.Mol.Biol.