CATH Classification

Domain Context

CATH Clusters

Superfamily Rieske [2Fe-2S] iron-sulphur domain
Functional Family Biphenyl/naphthalene dioxygenase alpha subunit

Enzyme Information

1.14.12.3
Benzene 1,2-dioxygenase.
based on mapping to UniProt A5W4F2
Benzene + NADH + O(2) = cis-cyclohexa-3,5-diene-1,2-diol + NAD(+).
-!- A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase and ferredoxin.
1.14.12.11
Toluene dioxygenase.
based on mapping to UniProt A5W4F2
Toluene + NADH + O(2) = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+).
-!- A system, containing a reductase which is an iron-sulfur flavoprotein (FAD), an iron-sulfur oxygenase, and a ferredoxin. -!- Some other aromatic compounds, including ethylbenzene, 4-xylene and some halogenated toluenes, are converted into the corresponding cis- dihydrodiols.

UniProtKB Entries (1)

A5W4F1
BNZB_PSEP1
Pseudomonas putida F1
Benzene 1,2-dioxygenase subunit beta

PDB Structure

PDB 3EN1
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system
Friemann, R., Lee, K., Brown, E.N., Gibson, D.T., Eklund, H., Ramaswamy, S.
Acta Crystallogr.,Sect.D