CATH Classification

Domain Context

CATH Clusters

Superfamily Phosphorylase Kinase; domain 1
Functional Family Dual-specificity mitogen-activated protein kinase kinase 4

Enzyme Information

2.7.12.2
Mitogen-activated protein kinase kinase.
based on mapping to UniProt P45985
ATP + a protein = ADP + a phosphoprotein.
-!- A dual-specific protein kinase and requires mitogen-activated protein kinase kinase kinase (MAPKKK) for activation. -!- It is required for activation of EC 2.7.11.24. -!- Phosphorylation of MEK1 by Raf involves phosphorylation of two serine residues. -!- Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. -!- Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumor necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischemeic injury.

UniProtKB Entries (1)

P45985
MP2K4_HUMAN
Homo sapiens
Dual specificity mitogen-activated protein kinase kinase 4

PDB Structure

PDB 3ALN
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structures of MKK4 kinase domain reveal that substrate peptide binds to an allosteric site and induces an auto-inhibition state
Matsumoto, T., Kinoshita, T., Kirii, Y., Yokota, K., Hamada, K., Tada, T.
Biochem.Biophys.Res.Commun.