CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
2 | Mainly Beta |
|
2.102 | 3-layer Sandwich |
|
2.102.10 | Rieske Iron-sulfur Protein |
|
2.102.10.10 | Rieske [2Fe-2S] iron-sulphur domain |
Domain Context
CATH Clusters
| Superfamily | Rieske [2Fe-2S] iron-sulphur domain |
| Functional Family | Biphenyl dioxygenase ferredoxin subunit |
Enzyme Information
| 1.-.-.- |
Oxidoreductases.
based on mapping to UniProt P71875
|
| 1.14.13.142 |
3-ketosteroid 9-alpha-monooxygenase.
based on mapping to UniProt P71875
Androsta-1,4-diene-3,17-dione + NADH + O(2) = 9-alpha-hydroxyandrosta- 1,4-diene-3,17-dione + NAD(+) + H(2)O.
-!- The enzyme is involved in the cholesterol degradation pathway of several bacterial pathogens, such as Mycobacterium tuberculosis. -!- It is a two-component system consisting of a terminal oxygenase (KshA) and a ferredoxin reductase (KshB). -!- The oxygenase contains a Rieske-type iron-sulfur center and non-heme iron. -!- The reductase component is a flavoprotein containing an NAD-binding domain and a plant-type iron-sulfur cluster. -!- The product of the enzyme is unstable, and spontaneously converts to 3-hydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione.
|
| 1.17.1.- |
With NAD(+) or NADP(+) as acceptor.
based on mapping to UniProt P71875
|
UniProtKB Entries (1)
| P71875 |
KSHA_MYCTU
Mycobacterium tuberculosis H37Rv
3-ketosteroid-9-alpha-monooxygenase, oxygenase component
|
PDB Structure
| PDB | 2ZYL |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Characterization of 3-ketosteroid 9{alpha}-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis
J.Biol.Chem.
|