CATH Classification

Domain Context

CATH Clusters

Superfamily Vaccinia Virus protein VP39
Functional Family Alpha N-terminal protein methyltransferase 1

Enzyme Information
Protein N-terminal methyltransferase.
based on mapping to UniProt Q9BV86
(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N- (A,S)PK-[protein]. (2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl- L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].
-!- This enzyme methylates the N-terminus of target proteins containing the N-terminal motif (Ala/Pro/Ser)-Pro-Lys after the initiator L-methionine is cleaved. -!- When the terminal amino acid is L-proline, the enzyme catalyzes two successive methylations of its alpha-amino group. -!- When the first amino acid is either L-alanine or L-serine, the enzyme catalyzes three successive methylations. -!- The Pro-Lys in positions 2-3 cannot be exchanged for other amino acids. -!- Formerly EC 2.1.1.n5.

UniProtKB Entries (1)

Homo sapiens
N-terminal Xaa-Pro-Lys N-methyltransferase 1

PDB Structure

External Links
Organism Escherichia
Primary Citation
The Crystal Structure of Human AD-003 protein in complex with S-adenosyl-L-homocysteine
Min, J., Wu, H., Zeng, H., Loppnau, P., Sundstrom, M., Arrowsmith, C.H., Edwards, A.M., Bochkarev, A., Plotnikov, A.N.
To be Published