CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome P450
Functional Family Fatty acid beta-hydroxylating cytochrome P450

Enzyme Information

1.11.2.4
Fatty-acid peroxygenase.
based on mapping to UniProt O31440
Fatty acid + H(2)O(2) = 3- or 2-hydroxy fatty acid + H(2)O.
-!- A cytosolic heme-thiolate protein with sequence homology to P450 monooxygenases. -!- Unlike the latter, it needs neither NAD(P)H, dioxygen nor specific reductases for function. -!- Enzymes of this type are produced by bacteria (e.g. Sphingomonas paucimobilis, Bacillus subtilis). -!- Catalytic turnover rates are high compared with those of monooxygenation reactions as well as peroxide shunt reactions catalyzed by the common P450s. -!- A model substrate is myristate, but other saturated and unsaturated fatty acids are also hydroxylated. -!- Oxidizes the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) and peroxygenates aromatic substrates in a fatty-acid-dependent reaction.

UniProtKB Entries (1)

O31440
CYPC_BACSU
Bacillus subtilis subsp. subtilis str. 168
Fatty-acid peroxygenase

PDB Structure

PDB 1IZO
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Substrate Recognition and Molecular Mechanism of Fatty Acid Hydroxylation by Cytochrome P450 from Bacillus subtilis. CRYSTALLOGRAPHIC, SPECTROSCOPIC, AND MUTATIONAL STUDIES.
Lee, D.S., Yamada, A., Sugimoto, H., Matsunaga, I., Ogura, H., Ichihara, K., Adachi, S., Park, S.Y., Shiro, Y.
J.Biol.Chem.