CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.720 | NAD(P)-binding Rossmann-like Domain |
Domain Context
CATH Clusters
| Superfamily | NAD(P)-binding Rossmann-like Domain |
| Functional Family | Malate dehydrogenase |
Enzyme Information
| 1.1.1.375 |
L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+)).
based on mapping to UniProt Q60176
A (2S)-2-hydroxycarboxylate + NAD(P)(+) = a 2-oxocarboxylate + NAD(P)H.
-!- The enzyme from the archaeon Methanocaldococcus jannaschii catalyzes the reversible oxidation of (2R)-3-sulfolactate and (S)-malate to 3-sulfopyruvate and oxaloacetate, respectively (note that (2R)-3- sulfolactate has the same stereochemical configuration as (2S)-2- hydroxycarboxylates). -!- The enzyme can use both NADH and NADPH, although activity is higher with NADPH. -!- The oxidation of (2R)-3-sulfolactate was observed only in the presence of NADP(+). -!- The same organism also possesses an NAD(+)-specific enzyme with similar activity, cf. EC 1.1.1.337.
|
UniProtKB Entries (1)
| Q60176 |
MDH_METJA
Methanocaldococcus jannaschii DSM 2661
L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+))
|
PDB Structure
| PDB | 1HYE |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.
J.Mol.Biol.
|