CATH Classification

Domain Context

CATH Clusters

Superfamily MutM-like, N-terminal
Functional Family Formamidopyrimidine-DNA glycosylase

Enzyme Information

4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt O50606
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.
3.2.2.23
DNA-formamidopyrimidine glycosylase.
based on mapping to UniProt O50606
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
-!- May play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents. -!- Also involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA.

UniProtKB Entries (1)

O50606
FPG_THET8
Thermus thermophilus HB8
Formamidopyrimidine-DNA glycosylase

PDB Structure

PDB 1EE8
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of a repair enzyme of oxidatively damaged DNA, MutM (Fpg), from an extreme thermophile, Thermus thermophilus HB8.
Sugahara, M., Mikawa, T., Kumasaka, T., Yamamoto, M., Kato, R., Fukuyama, K., Inoue, Y., Kuramitsu, S.
EMBO J.