CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.20
Functional Family D-alanine--D-alanine ligase

Enzyme Information

6.1.2.1
D-alanine--(R)-lactate ligase.
based on mapping to UniProt P25051
D-alanine + (R)-lactate + ATP = D-alanyl-(R)-lactate + ADP + phosphate.
-!- The product of this enzyme, the depsipeptide D-alanyl-(R)-lactate, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4. -!- The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.
6.3.2.4
D-alanine--D-alanine ligase.
based on mapping to UniProt P25051
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
-!- Involved with EC 6.3.2.7 or EC 6.3.2.13, EC 6.3.2.8, EC 6.3.2.9 and EC 6.3.2.10 in the synthesis of a cell-wall peptide.

UniProtKB Entries (1)

P25051
VANA_ENTFC
Enterococcus faecium
Vancomycin/teicoplanin A-type resistance protein VanA

PDB Structure

PDB 1E4E
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The molecular basis of vancomycin resistance in clinically relevant Enterococci: crystal structure of D-alanyl-D-lactate ligase (VanA).
Roper, D.I., Huyton, T., Vagin, A., Dodson, G.
Proc. Natl. Acad. Sci. U.S.A.