CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C12, ubiquitin carboxyl-terminal hydrolase
Functional Family Ubiquitin carboxyl-terminal hydrolase

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt P35127
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (2)

P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C
P35127
UBL1_YEAST
Saccharomyces cerevisiae S288C
Ubiquitin carboxyl-terminal hydrolase YUH1

PDB Structure

PDB 1CMX
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis for the specificity of ubiquitin C-terminal hydrolases.
Johnston, S.C., Riddle, S.M., Cohen, R.E., Hill, C.P.
EMBO J.