CATH Classification

Domain Context

CATH Clusters

Superfamily DNA-directed RNA polymerase, insert domain
Functional Family DNA-directed RNA polymerase subunit alpha

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt P0A7Z4
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (2)

P0A8V2
RPOB_ECOLI
Escherichia coli K-12
DNA-directed RNA polymerase subunit beta
P06223
RP54_KLEOX
Klebsiella oxytoca
RNA polymerase sigma-54 factor

PDB Structure

PDB 6GFW
External Links
Method ELECTRON MICROSCOPY
Organism
Primary Citation
Structures of Bacterial RNA Polymerase Complexes Reveal the Mechanism of DNA Loading and Transcription Initiation.
Glyde, R., Ye, F., Jovanovic, M., Kotta-Loizou, I., Buck, M., Zhang, X.
Mol. Cell