CATH Classification

Domain Context

CATH Clusters

Superfamily Queuine tRNA-ribosyltransferase-like
Functional Family Queuine trna-ribosyltransferase domain-containing protein

Enzyme Information

2.4.2.29
tRNA-guanine(34) transglycosylase.
based on mapping to UniProt P28720
(1) Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine. (2) Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7- carbaguanine(34) in tRNA + guanine.
-!- Certain prokaryotic and eukaryotic tRNAs contain the modified base queuine at position 34. -!- In eukaryotes queuine is salvaged from food and incorporated into tRNA directly via a base-exchange reaction, replacing guanine. -!- In eubacteria, which produce queuine de novo, the enzyme catalyzes the exchange of guanine with the queuine precursor preQ(1), which is ultimately modified to queuine. -!- The eubacterial enzyme can also use an earlier intermediate, preQ(0), to replace guanine in unmodified tRNA(Tyr) and tRNA(Asn). -!- This enzyme acts after EC 1.7.1.13 in the queuine-biosynthesis pathway.

UniProtKB Entries (1)

P28720
TGT_ZYMMO
Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Queuine tRNA-ribosyltransferase

PDB Structure

PDB 5I02
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Co-crystallization, Isothermal titration calorimetry and nanoESI-MS reveal dimer disturbing inhibitors
Ehrmann, F.R., Stojko, J., Heine, A., Diederich, F., Reuter, K., Sanglier-Cianferani, S., Klebe, G.
To be Published