CATH Classification

Domain Context

CATH Clusters

Superfamily P-loop containing nucleotide triphosphate hydrolases
Functional Family Reverse gyrase 1

Enzyme Information
DNA helicase.
based on mapping to UniProt O51934
ATP + H(2)O = ADP + phosphate.
-!- DNA helicases utilize the energy from ATP hydrolysis to unwind double-stranded DNA. -!- Some of them unwind duplex DNA with a 3' to 5' polarity (1,3,5,8), other show 5' to 3' polarity (10,11,12,13) or unwind DNA in both directions (14,15). -!- Some helicases unwind DNA as well as RNA (4,9). -!- May be identical with EC (RNA helicase).
DNA topoisomerase (ATP-hydrolyzing).
based on mapping to UniProt O51934
ATP-dependent breakage, passage and rejoining of double-stranded DNA.
-!- Can introduce negative superhelical turns into double-stranded circular DNA. -!- One unit has nicking-closing activity, and another catalyzes super- twisting and hydrolysis of ATP (cf. EC

UniProtKB Entries (1)

Thermotoga maritima MSB8
Reverse gyrase

PDB Structure

External Links
Primary Citation
Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.
Rudolph, M.G., Del Toro Duany, Y., Jungblut, S.P., Ganguly, A., Klostermeier, D.
Nucleic Acids Res.