CATH Classification

Domain Context

CATH Clusters

Superfamily Glycosidases
Functional Family

Enzyme Information

2.4.1.248
Cycloisomaltooligosaccharide glucanotransferase.
based on mapping to UniProt P94286
Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)- alpha-D-glucosidic bond.
-!- Specific for (1->6)-alpha-D-glucans (dextrans) and, unlike cyclomaltodextrin glucanotransferase (EC 2.4.1.19), without activity toward (1->4)-alpha-D-glucans, such as amylose. -!- It also has no activity on oligosaccharides, such as amylopectin and pullulan, containing (1->6)-alpha-D-glucosidic linkages at branch points. -!- The enzyme from Bacillus circulans T-3040 has been shown to form cycloisomalto-oligosaccharides of three sizes (7, 8 and 9 glucose units). -!- It will also catalyze the disproportionation of two isomalto- oligosaccharides molecules to yield a series of isomalto- oligosachharides and the addition of D-glucose to cycloisomalto- oligosaccharides with ring opening to form isomalto-oligosaccharides.

UniProtKB Entries (1)

P94286
CTA1_BACCI
Bacillus circulans
Cycloisomaltooligosaccharide glucanotransferase

PDB Structure

PDB 3WNO
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural elucidation of the cyclization mechanism of alpha-1,6-glucan by Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase.
Suzuki, N., Fujimoto, Z., Kim, Y.M., Momma, M., Kishine, N., Suzuki, R., Suzuki, S., Kitamura, S., Kobayashi, M., Kimura, A., Funane, K.
J.Biol.Chem.