CATH Classification

Domain Context

CATH Clusters

Superfamily Aldolase class I
Functional Family

Enzyme Information

4.1.3.44
tRNA 4-demethylwyosine synthase (AdoMet-dependent).
based on mapping to UniProt O59412
N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO(2) + H(2)O.
-!- This enzyme, which is a member of the superfamily of S-adenosyl-L- methionine-dependent radical (radical AdoMet) enzymes, binds two [4Fe-4S] clusters. -!- Carbons C(2) and C(3) from pyruvate are incorporated into 4-demethylwyosine. -!- The enzyme is found in eukaryotes where it is part of the pathway for wybutosine synthesis, and in archaea, where it is involved in the biosynthesis of archaeal wye bases, such as wyosine, isowyosine, and methylwyosine.

UniProtKB Entries (1)

O59412
TYW1_PYRHO
Pyrococcus horikoshii OT3
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

PDB Structure

PDB 2YX0
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis
Goto-Ito, S., Ishii, R., Ito, T., Shibata, R., Fusatomi, E., Sekine, S., Bessho, Y., Yokoyama, S.
Acta Crystallogr.,Sect.D